Thermoanaerobacter pseudethanolicus (ATCC® 33223)

Strain Designations: 39E  /  Type Strain: no  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Clostridium thermohydrosulfuricum Klaushofer and Parkkinen
Strain Designations 39E
Degrades starch
Produces ethyl alcohol ethanol
Produces glucoamylase
Produces pullulanase pullulan 6-glucanohydrolase
Produces thermostable pullulanase and glucoamylase
Produces ethanol from starch
Microbial mat
United States
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Preceptrol® no
Genome Sequenced Strain

Thermoanaerobacter pseudethanolicus ATCC 33223, complete genome.

Type Strain no
Genome sequenced strain
Medium ATCC® Medium 1118: Methanobacteria medium (ATCC medium 1045) with 0.2% yeast extract and 0.5% glucose
Growth Conditions
Temperature: 65.0°C
Atmosphere: Anaerobic
Name of Depositor JG Zeikus
Special Collection National Park Service Special Collection (NPS)
Microbial mat
United States
Geographical Isolation Yellowstone National Park

Hyun HH, Zeikus JG. General biochemical characterization of thermostable pullulanase and glucoamylase from Clostridium thermohydrosulfuricum. Appl. Environ. Microbiol. 49: 1168-1173, 1985.

Zeikus JG, Hyun HH. Co-culture production of thermostable enzymes and ethanol. US Patent 4,604,352 dated Aug 5 1986

Lee YE, et al. Taxonomic distinction of saccharolytic thermophilic anaerobes: description of Thermoanaerobacterium xylanolyticum gen. nov., sp. nov., and Thermoanaerobacterium saccharolyticum gen. nov., sp. nov.; reclassification of Thermoanaerobium brockii, Clostridium thermosulfurogenes, and Clostridium thermohydrosulfuricum E100-69 as Thermoanaerobacter brockii comb. nov., Thermoanaerobacterium thermosulfurigenes comb. nov., and Thermoanaerobacter thermohydrosulfuricus comb. nov., respectively; and transfer of Clostr. Int. J. Syst. Bacteriol. 43: 41-51, 1993.

algal mat, Yellowstone National Park

Cross References

Nucleotide (GenBank) : Y08557 T. ethanolicus melA and lacA genes.

Nucleotide (GenBank) : L09164 Thermoanaerobacter ethanolicus (ATCC 33223) 16S ribosomal RNA (16S rRNA) gene.

Nucleotide (GenBank) : U49975 Thermoanaerobacter ethanolicus secondary-alcohol dehydrogenase (adhB) gene, complete cds.

Nucleotide (GenBank) : E03575 DNA sequence encoding clostridium thermohydrosulfuricum thermo-resistant xylose isomerase.

Nucleotide (GenBank) : CP000924 Thermoanaerobacter pseudethanolicus ATCC 33223, complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • This material is distributed for scientific and educational research purposes only and is part of the National Park Service Special Collection. A signed National Park Service MTA is both required prior to shipment to customers proposing a non-commercial use. A separate agreement is required from the National Park Service for customers proposing a commercial use.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation