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D24 (ATCC® CRL-2701™)

Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte  /  Tissue: spleen  / 

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Organism Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)
Tissue spleen
Cell Type hybridoma:lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
ELISA detection colony stimulating factor 1 (macrophage)
Storage Conditions Complete growth medium supplemented with 5% (v/v) DMSO
Derivation
Animals were immunized with purified mouse colony stimulating factor-1 (CSF-1). Spleen cells were fused with Y3-Ag.1.2.3 myeloma cells. The D24 (ATCC CRL-2701) antibody binds to mouse CSF-1 but does not neutralize its biological activity, while the 5A1 (ATCC CRL-2702) antibody binds to and neutralizes the biological function of CSF-1 [PubMed: 2454994]. The D24 antibody (ATCC CRL-2701) can be used with the 5A1 antibody (ATCC CRL-2702) in ELISA assays to quantitate the amount of naturally occurring mouse CSF-1 [PubMed: 2677146]. These antibodies recognize different epitopes of the same antigen, thereby permitting the detection of low amounts of CSF-1 [PubMed: 2677146].
Genes Expressed
immunoglobulin; monoclonal antibody; against colony stimulating factor-1 (CSF-1),colony stimulating factor 1 (macrophage)
Cellular Products
immunoglobulin; monoclonal antibody; against colony stimulating factor-1 (CSF-1)
Tumorigenic Yes
Effects
Yes, ascites in pristane primed Lou/M rats
Comments
Animals were immunized with purified mouse colony stimulating factor-1 (CSF-1). Spleen cells were fused with Y3-Ag.1.2.3 myeloma cells. The D24 (ATCC CRL-2701) antibody binds to mouse CSF-1 but does not neutralize its biological activity, while the 5A1 (ATCC CRL-2702) antibody binds to and neutralizes the biological function of CSF-1 [PubMed: 2454994]. The D24 antibody (ATCC CRL-2701) can be used with the 5A1 antibody (ATCC CRL-2702) in ELISA assays to quantitate the amount of naturally occurring mouse CSF-1 [PubMed: 2677146]. These antibodies recognize different epitopes of the same antigen, thereby permitting the detection of low amounts of CSF-1 [PubMed: 2677146].
Complete Growth Medium Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: Complete growth medium supplemented with 5% (v/v) DMSO
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG1 and 2a
Name of Depositor H Lin
Deposited As rat (B cell); rat (myeloma)
Year of Origin July, 1988
References

Lokeshwar BL, Lin HS. Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor. J. Immunol. 141: 483-488, 1988. PubMed: 2454994

Lokeshwar BL, Lin HS. A sandwich enzyme-linked immunoadsorbent assay for detection of murine macrophage colony-stimulating factor (CSF-1). J. Immunol. Methods 123: 123-129, 1989. PubMed: 2677146

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Lokeshwar BL, Lin HS. Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor. J. Immunol. 141: 483-488, 1988. PubMed: 2454994

Lokeshwar BL, Lin HS. A sandwich enzyme-linked immunoadsorbent assay for detection of murine macrophage colony-stimulating factor (CSF-1). J. Immunol. Methods 123: 123-129, 1989. PubMed: 2677146