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Human adenovirus 19 (ATCC® VR-254)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus D  /  Product Format: frozen 1.0 mL

Permits and Restrictions

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Classification Adenoviridae, Mastadenovirus, Human adenovirus D
Deposited As Adenovirus type 19
Agent Human adenovirus 19
Strain AV-587 [3911]
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Comments
Replicates very slowly. Agglutinates rat RBC. Identical to adenovirus type 10 in HAI but different in neutralization tests
REFERENCE STRAIN: Pereira, H.G. et al., Virology 20: 613, 1963.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
KB cells (ATCC® CCL-17™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01-0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 6-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor SD Bell
Source Conjunctival scrapings from child with trachoma, Saudi Arabia, 1955
Year of Origin 1955
References

Bell SD. . Am. J. Trop. Med. Hyg. 9: 523, 1960.

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation