SUP-T1 [VB] (ATCC® CRL-1942)

Organism: Homo sapiens, human  /  Cell Type: T lymphoblast  /  Disease: T-cell lymphoblastic lymphoma

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Organism Homo sapiens, human
Cell Type T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease T-cell lymphoblastic lymphoma
Age 8 years
Gender male
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype 92, XXYY, -2, -2, -9, -9, the following markers are present: 2inv(2*) (p21q11), +2inv(2*), del(2*)(Q21), 2del(4)(q31q35), 2del(5)(p13p14), 2del(6)(q23q27), 2inv(14)(q11q32), t(7;9)(q34;q34), der(9)t(7;9)
Images CRL-1942 Micrograph
Derivation
This line was derived from malignant cells collected from the malignant pleural effusion of an 8 year old child with T-Cell Lymphoblastic Lymphoma.
Clinical Data
8 years
Caucasian
male
Antigen Expression

Leu-6 (CD1a) +; Leu-4 (CD3) +; Leu-3 (CD4) +; Leu-1 (CD5) +; Leu-9 (CD7) +; Leu-2a (CD8) +; OKT-10 (CD38) + RefSmith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672 RefHecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800 RefLifson JD, et al. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463 

Genes Expressed
immunoglobulin (cytoplasmic)
Cellular Products
immunoglobulin (cytoplasmic)
Comments
The cells express multiple T lineage markers.
The cells are E - rosette negative and beta 2 microglobulin positive.
The cells are negative for Leu 5 (CD2), sheep erythrocyte receptor, OKT 9 (CD71; transferrin receptor), surface immunoglobulin (sIg -), HLA DR, CALLA (CD10) and Tac (CD25).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 x 105 viable cells/mL and maintain between 2 x105 and 2 x 106 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor SD Smith
References

Smith SD, et al. Clinical and biologic characterization of T-cell neoplasias with rearrangements of chromosome 7 band q34. Blood 71: 395-402, 1988. PubMed: 2962650

Baer R, et al. Fusion of an immunoglobulin variable gene and a T cell receptor constant gene in the chromosome 14 inversion associated with T cell tumors. Cell 43: 705-713, 1985. PubMed: 3935328

Reynolds TC, et al. Analysis of DNA surrounding the breakpoints of chromosomal translocations involving the beta T cell receptor gene in human lymphoblastic neoplasms. Cell 50: 107-117, 1987. PubMed: 3036364

Smith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672

Hecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800

Lifson JD, et al. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Smith SD, et al. Clinical and biologic characterization of T-cell neoplasias with rearrangements of chromosome 7 band q34. Blood 71: 395-402, 1988. PubMed: 2962650

Baer R, et al. Fusion of an immunoglobulin variable gene and a T cell receptor constant gene in the chromosome 14 inversion associated with T cell tumors. Cell 43: 705-713, 1985. PubMed: 3935328

Reynolds TC, et al. Analysis of DNA surrounding the breakpoints of chromosomal translocations involving the beta T cell receptor gene in human lymphoblastic neoplasms. Cell 50: 107-117, 1987. PubMed: 3036364

Smith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672

Hecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800

Lifson JD, et al. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463