Simian virus 40 (ATCC® VRMC-31)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: frozen

Classification Polyomaviridae, Polyomavirus
Agent Simian virus 40
Strain pUCSV40-777-1 [molecular clone of Simian virus 40, SV-40]
Common Name Simian virus 40
Applications
contains sequence
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Comments
The insert contains the following restriction sites (kb): BamHI--4.449, 3.417; EcoRI--5.180, 2.686; KpnI--6.365, 1.501; SalI--7.866; SfiI--7.866; XhoI--uncut
Plasmid size: 7.866 kb [2.686 kb (pUC19 vector) + 5.180 kb (SV40-777-1 insert)]
Plasmid copy#: High-copy number (pUC19 has a ColEI ori)
Viable virus clone. Verified by lipofection of EcoRI-linearized plasmid DNA into ATCC CV-1 cells (passage# 40) by J. Lednicky (lipofection date: 12 Oct. 2000).
This construct is an archetypal-regulatory region variant of SV40-777.
Name of Depositor JA Lednicky
Special Collection NCRR Contract