NCI-H345 [H345] (ATCC® HTB-180)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: bone marrow  /  Disease: carcinoma; small cell lung cancer

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: bone marrow
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates and some adherent cells (This cell line grows as gland-like clusters in suspension with a few attached cells. The viability of the cells in clusters is better than the viability of the single cells in the cultures.)
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease carcinoma; small cell lung cancer
Age 64 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype hypotriploid; modal number = 63; range = 60 to 66.
Sixteen or more marker chromosomes were common to most cells. Nearly half of the markers had lengths close to the D group or smaller chromosomes. Among the identifiable markers were der(1)t(1;17)(q21;q11), der(?9)t(1;?9)()q12;?q21, t(8q?12q), t(12q?17q), der(9)t(9,?)(q22;?). Normal N13 was absent; the N8, N9, N12 and N19 were found as single copies; N20 generally had 4 copies; there were 2 normal X chromosomes and no Y chromosome was detected.
Derivation The NCI-H345 cell line was derived by D. Carney, A.F. Gazdar and associates in 1981 from the bone marrow of a patient with small cell carcinoma of the lung (SCLC). The tumor specimen was obtained after the initiation of chemotherapy.
Clinical Data
64 years
Caucasian
male
Cellular Products
gastrin releasing peptide
Tumorigenic Yes
Effects
Yes, in nude mice
Comments

The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue.

The line does not exhibit any gross structural DNA abnormalities.

The cells express elevated levels of four biochemical markers of SCLC: neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.

This cell line grows as gland-like clusters in suspension with a few attached cells. The viability of the cells in clusters is better than the viability of the single cells in the cultures.

The cells form transplantable tumors with typical small cell carcinoma histology.


Complete Growth Medium HITES medium (serum free) is formulated at the ATCC as follows:
  • DMEM: F-12 Medium (ATCC 30-2006)
  • Insulin 0.005mg/ml (Gibco 12585-014)
  • Transferrin 0.01 mg/ml (Sigma T5391 or equivalent)
  • Sodium selenite 30 nM (Sigma S9133 or equivalent)
  • Hydrocortisone 10 nM (Sigma H0135 or equivalent)
  • beta-estradiol 10 nM (Sigma E2257 or equivalent)
  • L-Glutamine Solution (ATCC 30-2214) 2 mM (in addition to that in the base medium)
Subculturing
Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium. Aggregates may be dispersed by trituration. The use of trypsin is not recommended.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 8
D5S818: 11,12
D7S820: 9,13
THO1: 9,9.3
TPOX: 8,9
vWA: 15,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1-2
PGM3, 1
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
Year of Origin 1981
References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893

This cell line grows as gland-like clusters in suspension with a few attached cells. The viability of the cells in clusters is better than the viability of the single cells in the cultures.

Basic Documentation
Restrictions

The line is available with the following restrictions:

  1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.
  2. Any proposed commercial use of the these cells, or their products, must first be negotiated with the National Cancer Institute (NCI).  For further information, please contact NCI’s Technology Transfer Center at NCI_TTC_Contact@mail.nih.gov or by phone at (240)-276-5514.

References

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893

This cell line grows as gland-like clusters in suspension with a few attached cells. The viability of the cells in clusters is better than the viability of the single cells in the cultures.