E.G7-OVA [derivative of EL4] (ATCC® CRL-2113)

Organism: Mus musculus, mouse  /  Cell Type: T lymphocyte  /  Tissue: lymphocyte  /  Disease: lymphoma

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Organism Mus musculus, mouse
Tissue lymphocyte
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain C57BL/6N
Applications
This cell line is a model system for studying major histocompatibility complex (MHC) class I restricted responses of cytotoxic T lymphocytes in mice.
Storage Conditions liquid nitrogen vapor phase
Derivation
E.G7-OVA was derived in 1988 from the C57BL/6 (H-2 b) mouse lymphoma cell line EL4 (ATCC TIB-39).
The EL4 cells were transfected by electroporation with the plasmid pAc-neo-OVA which carries a complete copy of chicken ovalbumin (OVA) mRNA and the neomycin (G418) resistance gene.
Antigen Expression
H-2 b
Genes Expressed
chicken ovalbumin
Comments
E.G7-OVA cells contain a single copy of the inserted plasmid and synthesize and secrete OVA constitutively.
C57BL/6 mice immunized with E.G7-OVA cells give rise to H-2 Kb restricted cytotoxic lymphocytes specific for the OVA 258-276 peptide.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol and 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor MJ Bevan
Year of Origin 1988
References

Moore MW, et al. Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell 54: 777-785, 1988. PubMed: 3261634

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Moore MW, et al. Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell 54: 777-785, 1988. PubMed: 3261634