N1-S1 Fudr (ATCC® CRL-1603)

Organism: Rattus norvegicus, rat  /  Tissue: liver  /  Disease: hepatoma; Novikoff hepatoma

Organism Rattus norvegicus, rat
Tissue liver
Product Format frozen
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease hepatoma; Novikoff hepatoma
Gender male
Strain Sprague-Dawley
Applications
The cells are resistant to 5-fluoro-2'-deoxyuridine (FUdR) at 0.05 mM and to 5-fluorouracil.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is derivative of N1-S1 (ATCC CRL-1604).
Clinical Data
male
Comments
The cells are resistant to 5-fluoro-2'-deoxyuridine (FUdR) at 0.05 mM and to 5-fluorouracil.
This line is derivative of N1-S1 (ATCC CRL-1604).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • 10% fetal bovine serum
  • 0.05 mM FUdR
  • 0.1% pluronic F68

Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 x 104 viable cells/mL and subculture at 1 x 106 cells/mL (about 3 days). Cells can be grown in Florence flasks on a rotary shaker at 180 to 190 RPM.
Medium Renewal: Twice per week
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor JE Becker
References

Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488

Cory JG, et al. Evidence for role of purine nucleoside phosphorylase in sensitivity of Novikoff hepatoma cells to 5-fluorouracil. Adv. Enzyme Regul. 15: 153-166, 1976. PubMed: 197803

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Basic Documentation
References

Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488

Cory JG, et al. Evidence for role of purine nucleoside phosphorylase in sensitivity of Novikoff hepatoma cells to 5-fluorouracil. Adv. Enzyme Regul. 15: 153-166, 1976. PubMed: 197803

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.