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8E5 [derivative of CEM] (ATCC® CRL-8993™)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast human immunodeficiency virus (HIV) positive  /  Tissue: peripheral blood  /  Disease: acute lymphoblastic leukemia

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type lymphoblast human immunodeficiency virus (HIV) positive
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Retrovirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease acute lymphoblastic leukemia
Age 4 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data female
Caucasian
4 years
Antigen Expression
CD4 +; CD5 +
Receptor Expression
transferrin
Genes Expressed
most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins
Cellular Products
most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins
Comments

The cells contain a single defective proviral genome of HTLV III (HIV, LAV).

All of the major structural protein are constitutively expressed with the exception of the p64 and p34 proteins.

No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation.

The cells have been cured of a mycoplasma contamination.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days.
Cryopreservation
Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Name of Depositor The United States of America
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Powell DM, et al. Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Powell DM, et al. Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.