Orientia chuto (ATCC® VR-1744)

Classification: Rickettsiaceae  /  Product Format: frozen 1 mL per vial

Classification Rickettsiaceae
Agent Orientia chuto
Strain Dubai
Biosafety Level 3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions Vapor phase of liquid nitrogen
Images VR-1744 images
Comments
New Taxon.
It is recommended to determine viability by a PCR-based assay as agent does not produce cytopathic effects.
Effect on Host
Toxicity observed
Recommended Host
Vero cells (ATCC® CCL-81™)
Growth Conditions

Temperature: 35°C

Recommendations for Infection: Plate cells 24-36 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI. Centrifuge at 800 x g at room temperature for 75 minutes - 2 hours. End adsorption by adding growth medium.

Incubation: 15 days

Effect on Host
Toxicity observed
Name of Depositor L Izzard, Australian Rickettsial Reference Laboratory
Source Clinical female patient with acute scrub typhus infection, Dubai, United Arab Emirates, 2006.
References

Izzard L, et. al. Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai. J. Clin. Microbiol. 48(12): 4404-4409, 2010. PubMed: 20926708