KG-1 (ATCC® CCL-246)

Organism: Homo sapiens, human  /  Cell Type: Macrophage  /  Tissue: bone marrow  /  Disease: acute myelogenous leukemia

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Organism Homo sapiens, human
Tissue bone marrow
Cell Type Macrophage
Product Format frozen
Morphology myeloblast
Culture Properties suspension
Biosafety Level 1
Disease acute myelogenous leukemia
Age 59 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype The stemline chromosome number is near-diploid, with the 2S component occurring at 1.8%. Five markers (constitutive markers) were common to most, if not all, metaphases analyzed. Modal chromosome number is 47 (or 46 plus a small metacentric chromosome which is smaller than the G1 group chromosome). Normal chromosomes 5, 7, 8, 12 and 17 were monosomic, and others were disomic. The Y chromosome is detected in the Q-banded preparations.
Derivation
The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde. A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia.
Clinical Data
59 years
Caucasian
male
Antigen Expression
HLA A30, A31, B35, Cw4
Genes Expressed
HLA DR
Comments
KG-1 cells spontaneously differentiate to granulocyte and macrophage like cells.
They show a good response to colony stimulating factor (CSF).
The line is EBNA negative (EBNA-).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL. Do not allow to exceed 2 x 106 cells/mL.
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Isoenzymes
AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1-2
PGM3, 0
Name of Depositor DW Golde
References

Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765

Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689

Hester JP et al. Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel. Blood 54(1): 254-268 1979. PubMed: 444670

Cross References

Nucleotide (GenBank) : AF146432 Homo sapiens cell-line KG-1a sodium/hydrogen exchanger isoform 1 mRNA, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765

Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689

Hester JP et al. Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel. Blood 54(1): 254-268 1979. PubMed: 444670