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Hybri-Care Medium (ATCC® 46-X)

Formulation Formulation
Product Format 1 Package (prepares 1L of medium)
Storage Conditions Store medium at 2°C to 8°C in the dark when not in use.
Applications
Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines.
A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.
Comments
Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines. Ingredients are essentially those of a modified DMEM and NCTC 135 plus insulin. A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

When used with 10% fetal bovine serum, Hybri-Care repeatedly has been found to support vigorous growth of most hybridomas in the ATCC collection.

Ozato, K., et al. J. Immunology 124: 533-540, 1980.
Biosafety Level N/A
Basic Documentation
Other Documentation
FAQs
  1. Filter Size to Use for Hybricare 46-X
    Hybricare medium (ATCC® 46-X ™) is sold as a powder.  After hydration, sodium bicarbonate must be added and the medium sterile filtered through a 0.22 micron filter....
    Date Updated: 6/16/2014
  2. The type of water used for preparing powdered media

    Many vendors sell powdered media that must be reconstituted. Hybricare, ATCC® 46-X ™ is one example.


    Date Updated: 9/10/2018