Human herpesvirus 5 (ATCC® VR-1590)

Classification: Herpesviridae, Cytomegalovirus  /  Product Format: frozen 1 mL per vial

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Classification Herpesviridae, Cytomegalovirus
Agent Human herpesvirus 5
Strain Merlin
Common Name Human cytomegalovirus
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Genome Sequenced Strain

Human herpesvirus 5 strain Merlin, complete genome

Comments
Low MOI applications, such as titration, may require incubation for as long as 3 weeks. This is a low passage strain of Human herpesvirus 5, deposited at ATCC® 4 passages from isolation. It retains the wild type complement of 165 genes, and contains no obvious mutations other than a single nucleotide substitution that truncates gene UL128. To retain genetic integrity, further passage should be limited.
Effect on Host
CPE, cell rounding and enlargement, cytoplasmic and nuclear inclusions
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and enlargement, cytoplasmic and nuclear inclusions
Name of Depositor GWG Wilkinson, Cardiff Public Health Laboratory
Source Urine from a congenitally infected infant, Cardiff, Wales
References

Davison A J, et al. Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes. J. Gen. Virol. 84 (Pt 3), 657-663 (2003). PubMed: 12604818

Dolan A, et al. Genetic content of wild-type human cytomegalovirus. Journal of General Virology, 85: 1301-1312, 2004. PubMed: 15105547

Cross References

Nucleotide (GenBank) : AY446894.2 Human herpesvirus 5 strain Merlin, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation