BB (ATCC® CCL-59)

Organism: Ictalurus nebulosus, bullhead, brown  / 

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Organism Ictalurus nebulosus, bullhead, brown
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 2 years
Virus Resistance
herpes simplex; poliovirus 2; vaccinia
Comments
BB cells will grow at 4 to 34C with an optimum of between 25 and 30C.
The cells do not do well when cultured on a glass surface.
Complete Growth Medium Minimum essential medium (Eagle) with 2mM L-glutamine and Hanks' BSS with 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove trypsin and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 23.0°C
Name of Depositor RG Malsberger
References

Wolf K, Quimby MC. Fish cell and tissue culture In: Wolf K, Quimby MC. Fish physiology, 3 New York Academic Press pp. 253-301, 1969

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Wolf K, Quimby MC. Fish cell and tissue culture In: Wolf K, Quimby MC. Fish physiology, 3 New York Academic Press pp. 253-301, 1969