lambda1059 (ATCC® 37474)

Applications: vector for constructing genomic librariesvector permitting positive selection for inserts  /  Depositors: S Brenner

Permits and Restrictions

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Designations lambda1059
Depositors S Brenner
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 45.7999992370605500
Vector: lambda1059 (phage, lambda - replacement)
Promoters: Promoter none
Construct size (kb): 45.79999923706055
Features: insert detection: Spi+
marker(s): ampR
promoter: none
replicon: lambda
vector for constructing genomic libraries
vector permitting positive selection for inserts
The vector is Spi+; recombinants are Spi-.
Replacement vector useful for library construction which accepts inserts of 6-24 kb.

Karn J, et al. Novel bacteriophage lambda cloning vector. Proc. Natl. Acad. Sci. USA 77: 5172-5176, 1980. PubMed: 6254062

Shipped freeze-dried