Bacillus subtilis (Ehrenberg) Cohn (ATCC® 47096)

Strain Designations: BG2864  /  Type Strain: no  /  Biosafety Level: 1

Strain Designations BG2864
Application
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype ΔaprE ΔnprE ΔflaA::kan
Type Strain no
Comments
This host has been used for transformation and screening of gene mutant libraries.
This strain was constructed by introducing the ΔflaA::kan mutation from strain PB5129 into strain BG2036.
Medium ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype ΔaprE ΔnprE ΔflaA::kan
Name of Depositor C Wang
References

Shafikhani S, et al. Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization. BioTechniques 23: 304-310, 1997. PubMed: 9266088