RPMI-1640 Medium (ATCC® 30-2001)

Formulation Formulation
Product Format 500 mL
Storage Conditions Store medium at 2°C to 8°C in the dark when not in use.
Applications RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
Comments
NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also checked for its ability to support the growth of several different cell lines using both sequential subcultures and plating efficiencies. Additional test results are listed in the Certificate of Analysis.

Test*

Specification

pH

7.0 to 7.4

Osmolality

246 to 306

mOsm/kg H2O

Sterility Testing

Pass

Endotoxin

≤ 0.5 EU/mL

Mycoplasma

Pass

Growth Promotion

Pass

*Please consult the Certificate of Analysis for lot-specific test results.


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