Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-525)

Strain Designations: ET12567  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations ET12567
Application
It is the key host strain for streptomyces workers worldwide.

This is a host for isolation of non-methylated DNA for transfer to other species.

This strain is used as a host to isolate DNA and plasmids for transformation into Streptomyces or any other organism that has a methylation-sensitive restriction system.

Isolation
Derived from existing strain, constructed from strain GM2929 by T. MacNeil
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype F- dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44
Type Strain no
Medium ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype F- dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44
Name of Depositor DJ MacNeil, T MacNeil
Special Collection NSF - Bacteriology
Isolation
Derived from existing strain, constructed from strain GM2929 by T. MacNeil
References

MacNeil DJ, et al. Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111: 61-68, 1992. PubMed: 1547955

Douglas J MacNeil, personal communication