Waddlia chondrophila Everett et al. ATCC ® VR-1470™

Permits and Restrictions	View Permits
Classification	Waddliaceae
Agent	Waddlia chondrophila Everett et al.
Strain	WSU 86-1044
Applications	Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate). Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp. Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496.
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	pooled liver/lung homogenate from the aborted fetus of a 19-month-old primigravid Holstein cow during month 3 of gestation
Product Format	frozen
Storage Conditions	-70°C or colder

Genome Sequenced Strain	Yes
Comments	Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp. and 14 members of the genus Rickettsia all failed to react significantly. Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate). Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496. MEM with 10% serum
Effect on Host	Dense forms enter cytoplasm and reticulated forms develop, then change to dense forms and are released from the cell Inclusions visualized by light microscopy as dense forms. Some cell death due to lysis. Cells become rounded and dense.

Recommended Host	bovine turbinate (BT) cells (ATCC CRL-1390) BT cells (ATCC CRL-1390); P388D1 mouse macrophage cells (ATCC CCL-46)
Growth Conditions	Incubation: 3 days at 35°C in BT cells (ATCC CRL-1390), 5% CO₂

Effect on Host	Dense forms enter cytoplasm and reticulated forms develop, then change to dense forms and are released from the cell Inclusions visualized by light microscopy as dense forms. Some cell death due to lysis. Cells become rounded and dense.

Name of Depositor	TF McElwain
Special Collection	NCRR Contract
Source	pooled liver/lung homogenate from the aborted fetus of a 19-month-old primigravid Holstein cow during month 3 of gestation
References	Dilbeck PM, et al. Isolation of a previously undescribed rickettsia from an aborted bovine fetus. J. Clin. Microbiol. 28: 814-816, 1990. PubMed: 2185269 Kocan KM, et al. Development of a rickettsia isolated from an aborted bovine fetus. J. Bacteriol. 172: 5949-5955, 1990. PubMed: 2211519 Rurangirwa FR, et al. Analysis of the 16S rRNA gene of micro-organism WSU 86-1044 from an aborted bovine foetus reveals that it is a member of the order Chlamydiales: proposal of Waddliaceae fam. nov., Waddlia chondrophila gen. nov., sp. nov.. Int. J. Syst. Evol. Microbiol. 49: 577-581, 1999. PubMed: 10319478 type strain Terry F McElwain, personal communication
Cross References	Nucleotide (GenBank) : AF042496 16S ribosomal RNA and 23S ribosomal RNA, complete sequence

Permits	These permits may be required for shipping this product: USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit. Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS

Permits

These permits may be required for shipping this product:

USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Basic Documentation