BCL2 Jurkat (ATCC® CRL-2899™) Organism: Homo sapiens, human / Cell Type: T Cell/ T Lymphocyte / Tissue: peripheral blood / Disease: acute T cell leukemia General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Homo sapiens, human Tissue peripheral blood Cell Type T Cell/ T Lymphocyte Product Format frozen Morphology lymphoid-like Culture Properties suspension Biosafety Level 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease acute T cell leukemia Age 14 years Gender male Applications This cell line over-expresses BCL-2, and is useful to study cell apoptosis, BCL-2 function and its pathway. Storage Conditions liquid nitrogen vapor phase Images Derivation The BCL2 Jurkat cell line was derived by transfecting human Jurkat T cells with the pSFFV-neo mammalian expression vector containing the human BCL-2 ORF insert and a neomycin-resistant gene (Addgene # 3349). Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days (1). Cellular Products Neomycin resistance gene, expressed BCL2 over-expressed, verified at ATCC Tumorigenic Yes Comments The BCL2 Jurkat cell line was derived by transfecting human Jurkat T cells with the pSFFV-neo mammalian expression vector containing the human BCL-2 ORF insert and a neomycin-resistant gene (Addgene # 3349). Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days (1). The BCL2 Jurkat cell line produces over-expression of human BCL-2. Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. An inoculum of 2 x 105 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is 2 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended. Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cryopreservation Freeze medium: 90% Fetal Bovine Serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% STR Profile D5S818: 9 D13S317: 8, 12 D7S820: 8, 12 D16S539: 11 vWA: 18, 19 TH01: 6, 9.3 Amelogenin: X Y TPOX: 8, 10 CSF1PO: 11, 12 Name of Depositor Stanley Korsmeyer Year of Origin 2003 References Yamamoto K, et al. BCL-2 is Phosphorylated and Inactivated by an ASK1/Jun N-Terminal Protein Kinase Pathway normally Activated at G2/M. Mol Cell Biol. Dec;19(12):8469-78. 1999. PubMed: 10567572 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS Other Documentation Cell Micrograph References Yamamoto K, et al. BCL-2 is Phosphorylated and Inactivated by an ASK1/Jun N-Terminal Protein Kinase Pathway normally Activated at G2/M. Mol Cell Biol. Dec;19(12):8469-78. 1999. PubMed: 10567572