REC-1 (ATCC® CRL-3004)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast  /  Disease: Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)

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Organism Homo sapiens, human
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)
Age 57 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a near-diploid cell line of male origin with a modal chromosome number of 45 and a polyploidy rate of 10%. The karyotype is highly rearranged with most of the cells containing the t(11;14)(q13;q32) common to mantle cell lymphoma. Also, there are approximately 5-6 derivative chromosomes in the karyology that have been described by others. Note: Cytogenetic information is based on initial seed stock at ATCC.
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Derivation
The human B-cell line Rec-1 was established from a t(11;14)(q13;q32) bearing lymphoma. RefRimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653
Clinical Data
male
Antigen Expression
CD3-, CD5-, CD10-, CD19+, CD20+, CD23-, FMC7- (verified at ATCC)
Oncogene p53
Genes Expressed
p53,CD3-, CD5-, CD10-, CD19+, CD20+, CD23-, FMC7- (verified at ATCC)
Comments
Cyclin D1 is overexpressed. RefSalaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 
An ongoing level of chromosome rearrangements is observed. RefCamps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697   
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL. Maintain cultures at a cell concentration between 3 X 105 and 1 X 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

 

Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Interval: Subculture when cell concentration is between 3x105 and 1x106 cells/mL.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: fetal bovine serum, 60%; RPMI-1640 Medium, 30%; DMSO, 10%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10
D16S539: 11
D5S818: 12,13
D7S820: 10,11
THO1: 9,9.3
TPOX: 8,9
vWA: 17
Population Doubling Time approximately 38 hours
Name of Depositor E. Campo
Year of Origin 2003
References

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

Rimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653

Pinyol M, et al. Inactivation of RB1 in mantle-cell lymphoma detected by nonsense-mediated mRNA decay pathway inhibition and microarray analysis. Blood: 109(12):5422-5429, 2007. PubMed: 17332242

Tagawa H, et al. Genome-wide array-based CGH for mantle-cell lymphoma: identification of homozygous deletions of the proapoptotic gene BIM. Oncogene, 1-11, 2004

Drexler HG, MacLeod RA. Malignant hematopoietic cell lines: in vitro models for the study of mantle cell lymphoma. Leuk. Res. 26: 787-787, 2002. PubMed: 12127550

REC-1 Product Information, DSMZ (German Collection of Microorganisms and Cell Cultures). Retreived July 09, 2007, from http://www.dsmz.de/index.htm.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

Rimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653

Pinyol M, et al. Inactivation of RB1 in mantle-cell lymphoma detected by nonsense-mediated mRNA decay pathway inhibition and microarray analysis. Blood: 109(12):5422-5429, 2007. PubMed: 17332242

Tagawa H, et al. Genome-wide array-based CGH for mantle-cell lymphoma: identification of homozygous deletions of the proapoptotic gene BIM. Oncogene, 1-11, 2004

Drexler HG, MacLeod RA. Malignant hematopoietic cell lines: in vitro models for the study of mantle cell lymphoma. Leuk. Res. 26: 787-787, 2002. PubMed: 12127550

REC-1 Product Information, DSMZ (German Collection of Microorganisms and Cell Cultures). Retreived July 09, 2007, from http://www.dsmz.de/index.htm.