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Porcine respiratory coronavirus (ATCC® VR-2384™)

Classification: Coronaviridae, Alphacoronavirus, Alphacoronavirus 1  /  Product Format: frozen 1.0 mL

Classification Coronaviridae, Alphacoronavirus, Alphacoronavirus 1
Agent Porcine respiratory coronavirus
Strain AR310
Applications
This strain provides a biological pure culture of a novel pathogenic porcine respiratory coronavirus (PRCV) and a vaccine derived therefrom effective against PRCV infection and transmissible gastroenteritis virus (TGEV) infection.
The virus produces moderate pneumonia in pigs, there is no indication of human health hazard.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images ATCC VR-2384 images
Comments

This strain provides a biological pure culture of a novel pathogenic porcine respiratory coronavirus (PRCV) and a vaccine derived therefrom effective against PRCV infection and Transmissible gastroenteritis virus (TGEV) infection.  The virus produces a moderate pneumonia in pigs.  There is no indication of human health hazard.

This material is cited in U.S. and/or other patent # 5,419,907 May 30, 1995.

Effect on Host
CPE, fused and rounded cells with sloughing
Recommended Host
ST cells (ATCC® CRL-1746™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 2-3 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, fused and rounded cells with sloughing
Name of Depositor Iowa State University Research Foundation
U.S. Patent
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Special Collection ATCC
Source Culture of a novel TGEV-related Porcine respiratory coronavirus
References

Paul PS, et al. Pathogenic porcine respiratory coronavirus. US Patent 5,419,907 dated May 30 1995