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Vector Information
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Size (kb): 5.6160001754760740 Vector: pRS424 (phagemid) Promoters: Promoter T7 (phi10) Construction: pJS92, pRS304, pBluescript II SK+ Marker(s):TRP1,ampR Construct size (kb): 5.616000175476074 Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: T3
promoter: T7 (phi10)
promoter: lac
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
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Applications
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YE-type (episomal) shuttle vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; PvuII--5.2, 0.5; XbaI--3.3, 1.4, 1.0. Contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 1.5 + or - 1.7% of progeny per doubling. YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. The order of the major features in this plasmid is: ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori(2 micron) - TRP1.
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References
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Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568
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Related Products
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Shipping Information
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Frozen glycerol stock of Escherichia coli containing the phagemid.
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