1G10 (ATCC® CRL-2223)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Lymphoma

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Lymphoma
Age adult
Strain Lewis
Applications
The antibody will neutralize the B7.1 antigen, but does not react with the B7.2 antigen.
Derivation
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse B7.1
Comments
Animals were immunized in the hind footpad with irradiated 5C2 mouse B cell lymphoma cells that had been cultured in the presence of dibutyryl-cAMP.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody will neutralize the B7.1 antigen, but does not react with the B7.2 antigen.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG2a
Name of Depositor SE Buchholz
References

Powers GD, et al. Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell. Immunol. 153: 298-311, 1994. PubMed: 7509723

Nabavi N, et al. Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression. Nature 360: 266-268, 1992. PubMed: 1279442

Chen C, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J. Immunol. 152: 2105-2114, 1994. PubMed: 7510738

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Powers GD, et al. Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell. Immunol. 153: 298-311, 1994. PubMed: 7509723

Nabavi N, et al. Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression. Nature 360: 266-268, 1992. PubMed: 1279442

Chen C, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J. Immunol. 152: 2105-2114, 1994. PubMed: 7510738