Human coxsackievirus A3 (ATCC® VR-1799)

Classification: Picornaviridae, Enterovirus, Enterovirus A  /  Product Format: frozen 1 mL per vial

Classification Picornaviridae, Enterovirus, Enterovirus A
Agent Human coxsackievirus A3
Strain Olson
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images ATCC VR-1799 Photomicrographs
Comments Derived by adaptation from human amnion cell product ATCC® VR-163™ to RD cells (ATCC® CCL-136™). ATCC® VR-163™ and ATCC® VR-1799™ have not been compared for sequence or infectivity.
Effect on Host
CPE, rounding and sloughing
Recommended Host
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, rounding and sloughing
Name of Depositor HA Wenner, University of Kansas Medical Center; ATCC
Source Stool of 2 year old with nuchal rigidity and hamstring muscle spasm, New York, 1948

Sickles GM, Dalldorf G. Serologic differences amoung strains of the coxsackie group of viruses. Proc. Soc. Exp. Biol. Med. 72(1): 30, 1949. PubMed: 15391667