Cells are immortalized MEFs which generated from BAX and BAK double knockout mice.
Cells are immortalized MEFs which generated from BAX and BAK double knockout mice (genotype: bax-/-, bak-/-)
BAX and BAK are pro-apoptotic BCL-2 family members. Cells are completely resistant to tBID-induced cytochrome c release and apoptosis.
Cells are resistant to multiple apoptotic stimuli that act through disruption of mitochondrial function: staurosporine, ultraviolet radiation, growth factor deprivation, etoposide, and the endoplasmic reticulum stress stimuli thapsigargin and tunicamycin.
Cells are resistant to Bim and Bad expression induced apoptosis.
Soft agar focus-formation assay revealed oncoprotein Ras and the adenovirus-5 E1A induced focus formation.
This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, BAX and BAK function, as well as cells that are resistant to multiple apoptotic stimuli.