Primary Subcutaneous Pre-adipocytes; Normal, Human (ATCC® PCS-210-010)

Organism: Homo sapiens, human  /  Tissue: Mature adipose tissue  / 

Organism Homo sapiens, human
Tissue Mature adipose tissue
Morphology Spindle-shaped, fibroblast-like
Growth Properties Adherent
Biosafety Level 1
Age Adult
Gender Lot-specific
Ethnicity Lot-specific
Applications
Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines
Product Format frozen 1 mL
Storage Conditions -130°C or below
Comments
ATCC® Human Pre-Adipocytes are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.
Complete Growth Medium
  1. Obtain one Fibroblast Growth Kit-Low Serum from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. 
  3. Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.
  6. Table 1. Fibroblast Growth Kit-Low Serum Components (ATCC® PCS-201-041)

    Component

    Volume

    Final Concentration

    rh FGF β

    0.5 mL

    5 ng/mL

    L-glutamine

    18.75 mL

    7.5 mM

    Ascorbic acid

    0.5 mL

    50 μg/mL

    Hydrocortisone hemisuccinate

    0.5 mL

    1 μg/mL

    rh Insulin

    0.5 mL

    5 μg/mL

    Fetal Bovine Serum

    10.0 mL

    2%

    Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 2.

    Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

    Component

    Volume

    Final Concentration

    Gentamicin-Amphotericin B Solution

    0.5 mL

    Gentamicin: 10 µg/mL

    Amphotericin B: 0.25 µg/mL

    Penicillin-Streptomycin-Amphotericin B Solution

    0.5 mL

    Penicillin: 10 Units/mL

    Streptomycin: 10 µg/mL

    Amphotericin B: 25 ng/mL

    Phenol Red

    0.5 mL

    33 µM


  7. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
  8. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for 30 days.
Volume 1 mL
Sterility Tests
Negative for bacteria, yeast, fungi
Negative for Mycoplasma
Viral Testing
Negative for HIV-1, HIV-2, Hepatitis-B and Hepatitis-C.
C of A
Certificate of Analysis
Certificate of Analysis
Basic Documentation
FAQs
  1. PCS-210-010 Pre-adipocytes

    The PCS-210-010 cells are derived from white adipose tissue.  It is very difficult to obtain brown adipose tissue from an adult.


    Date Updated: 12/27/2013