Canid herpesvirus 1 (ATCC® VR-552)

Classification: Herpesviridae, Varicellovirus  /  Product Format: frozen 1.0 mL

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Classification Herpesviridae, Varicellovirus
Deposited As Canine herpesvirus
Agent Canid herpesvirus 1
Strain D 004
Common Name Canine herpesvirus
Biosafety Level 2
Isolation
Respiratory tract of a dog
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-552 images
Comments No cross-neutralization with other herpesviruses. Can only be propagated in cells of canine origin. Purified 1 time by terminal dilution, and 3 times by plaque passage.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
MDCK cells (ATCC® CCL-34™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor LN Binn, Division of Veterinary Medicine, Walter Reed Army Institute of Research
Source Respiratory tract of a dog
References

Binn LN, et al. Viruses recovered from laboratory dogs with respiratory disease. Proc. Soc. Exp. Biol. Med. 126: 140-145, 1967. PubMed: 4294430

Cross References

Nucleotide (GenBank) : U63459 Canine herpesvirus DNA polymerase gene, partial cds.

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