Canid herpesvirus 1 (ATCC® VR-552)

Classification: Herpesviridae, Varicellovirus  /  Product Format: frozen 1.0 mL

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Classification Herpesviridae, Varicellovirus
Deposited As Canine herpesvirus
Agent Canid herpesvirus 1
Strain D 004
Common Name Canine herpesvirus
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Respiratory tract of a dog
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-552 images
Comments No cross-neutralization with other herpesviruses. Can only be propagated in cells of canine origin. Purified 1 time by terminal dilution, and 3 times by plaque passage.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
MDCK cells (ATCC® CCL-34™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor LN Binn, Division of Veterinary Medicine, Walter Reed Army Institute of Research
Source Respiratory tract of a dog
References

Binn LN, et al. Viruses recovered from laboratory dogs with respiratory disease. Proc. Soc. Exp. Biol. Med. 126: 140-145, 1967. PubMed: 4294430

Cross References

Nucleotide (GenBank) : U63459 Canine herpesvirus DNA polymerase gene, partial cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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