Universal Mycoplasma Detection Kit (ATCC® 30-1012K)


The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity. 

The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.

Component Volume Composition Storage
Lysis Buffer 2 mL Lytic agent + digestive enzymes -20°C
Universal PCM Mix 0.8 mL Proprietary mix of buffers, dNTPs, thermostable polymerase -20°C
Universal PCR Primers 0.1 mL Proprietary mix of universal forward and reverse primers -20°C
Sample Lysis Tubes 40 each 2 mL snap cap tubes, tight seal -20°C
Positive Control 50 µL 1 pg/µL pUC19::M. arginini target in TE -20°C

Type Strain no
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 40 assays
Storage Conditions -20°C
Basic Documentation
Other Documentation
  1. Universal Mycoplasma Detection Kit - Differences from former Mycoplasma Detection Kit
    Date Updated: 3/27/2014
  2. Universal Mycoplasma Detection Kit - Sensitivity
    The kit detects less than 20 genomes of
    Date Updated: 3/27/2014
  3. Universal Mycoplasma Detection Kit - Species Detected

    Date Updated: 3/27/2014

  4. Universal Mycoplasma Detection Kit - Time to Obtain Results
    Total time from sample preparation to results is less than 3 hours.
    Date Updated: 3/27/2014
  5. Universal Mycoplasma Detection Kit - Identifying Contaminant Species
    A positive mycoplasma results in a specific band following agarose gel electrophoresis. If speciation is required, the band can be excised from the gel and sequenced. This will identify the ...
    Date Updated: 3/27/2014
  6. Universal Mycoplasma Detection Kit - Species Recognized
    The assay detects over 60 unique strains of mycoplasma including the eight species most likely to afflict cell cultures:
    Date Updated: 3/27/2014
  7. Universal Mycoplasma Detection Kit - Testing Cell Culture Supernatant

    Date Updated: 3/27/2014

  8. Universal Mycoplasma Detection Kit - Testing Media
    Date Updated: 3/27/2014
  9. Universal Mycoplasma Detection Kit - Testing Adherent or Suspension Cells
    Either adherent or suspension cells can be tested. Adherent cells should be harvested at 50-70% confluency and suspension cells should be harvested in log-phase growth.
    Date Updated: 3/27/2014
  10. Universal Mycoplasma Detection Kit - Antibiotics in Medium
    Date Updated: 3/27/2014
  11. Universal Mycoplasma Detection Kit - Storing Samples from Interrupted Procedure
    Universal Mycoplasma Detection Kit - Taq DNA Polymerase
    Universal Mycoplasma Detection Kit - PCR for Mycoplasma Detection
    Date Updated: 3/27/2014
  12. Universal Mycoplasma Detection Kit - Primers
    Universal forward and reverse primers are a component of the kit. The primers target the highly conserved 16S rRNA region of the mycoplasma genome and generate a specific PC...
    Date Updated: 3/27/2014
  13. Universal Mycoplasma Detection Kit - Reaction Tubes
    Universal Mycoplasma Detection Kit - Not Using Touchdown PCR
    If you use regular PCR instead of the touchdown procedure, non-specific amplification may occur and bands may be present th...
    Date Updated: 3/27/2014
  14. Universal Mycoplasma Detection Kit - Touchdown Protocol
    Universal Mycoplasma Detection Kit - Controls
    A positive control (pUC19::M. arginini target) is included to verify that the PCR reaction was successful. A negative control is prepared by combining the Universal...
    Date Updated: 3/27/2014
  15. Universal Mycplasma Detection Kit - Skipping Inactivation Step
    The assay will NOT work. The protease in the Lysis Mix must be inactivated prior to adding the lysate to the PCR mixture.
    Date Updated: 3/27/2014