pGW10 (40167)

Applications: expression vector  /  Depositors: New England Biolabs, Inc., GG Wilson, New England Biolabs, Inc.

Permits and Restrictions

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Designations pGW10
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors New England Biolabs, Inc., GG Wilson, New England Biolabs, Inc.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 11.5609998703002900
Vector: pGW10 (plasmid)
Promoters: Promoter lambda PL
Construction: pBR322, bacteriophage lambda
Marker(s):ampR,tetR
Construct size (kb): 11.56099987030029
Features: marker(s): ampR, tetR
promoter: lambda PL, lambda PR
replicon: pMB1
repressor gene: cI857
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.2, 4.8, 0.85, 0.5; BamHI--6.8, 4.3; EcoRI--6.8, 4.3; PvuII--11.6.
The insert contains the following restriction sites (approximate kb from the 5' HindIII site): BamHI--2.5; NsiI--2.9, 9.5; ScaI--7.9; EcoRI--8.0.
The plasmid contains the following restriction sites (starting at the EcoRI site proceeding through the pBR322 sequences): HindIII--29, 5192, 9997, 10561, 10686; BamHI--375, 7601; SalI--651; PvuII--2066; PvuI--3735, 8889; EcoRI--1, 4361.
Expression vector encoding cI857.
Distributed in aliquots of 2 ug (40 ng/ul).
The 4.3 kb BamHI fragment contains the PL and PR promoters, the cI857 gene, and 375 bp of pBR322 sequence.
The order of the major features in this plasmid is: EcoRI site - tetR - pMB1 ori - bla - bacteriophage lambda sequences Q, N, PR, rex, cI857, PR, Cr, O.
References

Chen S, Wilson GG. Method for producing the AccI restriction endonuclease and methylase. US Patent 5,004,691 dated Apr 2 1991

Geoffrey G Wilson, personal communication

Shipped frozen
Product Sheet
Product Sheet
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