Designation / Description PGW10 PLASMID DNA
U.S. Patent Number
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): HindIII--5.2, 4.8, 0.85, 0.5; BamHI--6.8, 4.3; EcoRI--6.8, 4.3; PvuII--11.6.
The insert contains the following restriction sites (approximate kb from the 5' HindIII site): BamHI--2.5; NsiI--2.9, 9.5; ScaI--7.9; EcoRI--8.0.
The plasmid contains the following restriction sites (starting at the EcoRI site proceeding through the pBR322 sequences): HindIII--29, 5192, 9997, 10561, 10686; BamHI--375, 7601; SalI--651; PvuII--2066; PvuI--3735, 8889; EcoRI--1, 4361.
Expression vector encoding cI857.
Distributed in aliquots of 2 ug (40 ng/ul).
The 4.3 kb BamHI fragment contains the PL and PR promoters, the cI857 gene, and 375 bp of pBR322 sequence.
The order of the major features in this plasmid is: EcoRI site - tetR - pMB1 ori - bla - bacteriophage lambda sequences Q, N, PR, rex, cI857, PR, Cr, O.
Shipped frozen
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Basic Documentation

Chen S, Wilson GG. Method for producing the AccI restriction endonuclease and methylase. US Patent 5,004,691 dated Apr 2 1991

Geoffrey G Wilson, personal communication