B6/BLU (ATCC® SCRC-1019)

Organism: Mus musculus, mouse  /  Cell Type: embryonic stem cell  /  Tissue: inner cell mass  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue inner cell mass
Cell Type embryonic stem cell
Product Format frozen
Morphology stem cell
Biosafety Level 1
Age embryo
Gender male
Strain C57BL/6Tac
Storage Conditions liquid nitrogen vapor phase
Karyotype 40 XY, diploid
Derivation
The B6/BLU ES cell line was derived from a C57BL/6 transgenic line that contains a LacZ reporter. 
Clinical Data
male
Comments

This mouse ES cell line has been shown to be germline competent. 

The B6/BLU ES cell line was derived from a C57BL/6 transgenic line that contains a LacZ reporter. The transgene is a -globin LacZ fusion expressed exclusively in peripheral red blood cells. The transgene was assembled in pUC19, and contains the 1.9 kb KpnI-PvuII human 54 HS-2 fragment, upstream from the marked ß-globin transgene. The 5' part of the human ß-globin gene was fused to a 3' kb NcoI-BglII fragment obtained from pLacD. The 3' part of the -globin gene consisted of a 2.8 kb BamHI-XbaI fragment that contains the 3' end of exon 2, intron 2, exon 3 and 3' flanking sequence. 5' HS-2 was inserted in the genomic (5' to 3') orientation with respect to the transgene. The transgene was isolated from the plasmid vector backbone by cleavage with XhoI and SalI . Instead of determining chimerism visually by coat color or assessing chimerism genotypically in a tail DNA, chimerism is assessed quantitatively in the mesoderm by assessing the LacZ expression in a single drop of tail blood obtained at weaning.

Complete Growth Medium Grow ES cells in Mouse ES Cell Basal Medium (ATCC SCRR-2011) that has been supplemented with the following components:
1. 0.1 mM 2-mercaptoethanol (Life Technologies Cat. No. 21985-023)
2. 1,000 U/mL mouse leukemia inhibitory factor (LIF) (Millipore Cat. No. ESG1107)
3. 10% to 15% ES-Cell Qualified FBS (ATCC® SCRR-30-2020) or an ES cell qualified serum replacement
Complete Growth Medium for Mouse ES Cells is stable for 14 days when stored at 2°C to 8°C.
Complete Growth Medium
Grow ES cells in Mouse ES Cell Basal Medium (ATCC SCRR-2011) that has been supplemented with the following components:
0.1 mM 2-mercaptoethanol (Invitrogen Cat. No. 21985)
1,000 U/ml mouse leukemia inhibitory factor (LIF) (EMD Millipore Cat. No. ESG1107)
15% FBS, ES Cell Qualified (ATCC SCRR-30-2020)
Complete Growth Medium for Mouse ES Cells is stable for 14 days when stored at 2°C to 8°C. This medium is formulated for use with a 5% CO2 in air atmosphere.
Subculturing Subculturing Procedure

Note: To insure the highest level of viability, pre-warm media and Trypsin/EDTA to 37ºC before adding to cells. Volumes used in this protocol are for T75 flasks. Proportionally adjust the volumes for culture vessels of other sizes. A split ratio of 1:4 to 1:7 is recommended.

Feeder Cell Preparation for Subcultures

  1. Daily maintain a sufficient number of flasks that have been pre-plated with MEFs in complete medium for feeder cells.
  2. One hour before subculturing the ES cells, perform a 100% medium change for the MEFs using complete growth medium for ES cells.

Dissociation and Transfer of ES Cells

  1. Aspirate the medium from the flask(s) containing ES cells.
  2. Wash with PBS Ca+2/Mg+2-free (ATCC® SCRR-2201).
  3. Add 3.0 mL of 0.25% (w/v) Trypsin / 0.53 mM EDTA solution (ATCC® 30-2101) and place in incubator. After about one minute the ES colonies will dissociate and all cells will detach from the flask.
  4. Dislodge the cells by gently tapping the side of the flask then wash the cells off with 7-10 mL of fresh culture medium. Triturate cells several times with a 10 mL pipette in order to dissociate the cells into a single-cell suspension.
  5. Spin the cells at 270 x g for 5 min. Aspirate the supernatant.
  6. Resuspend in enough complete growth medium for ES cells to reseed new vessels at the desired split ratio (i.e. a split ratio of 1:4 to 1:7 is recommended). Perform a cell count to determine the total number of cells. ES cells should be plated at a density of 30,000 – 50,000 cells/ cm2.
  7. Add separate aliquots of the cell suspension to the appropriate size flask containing feeder cells and add an appropriate volume of fresh complete growth medium for ES cells to each vessel.
  8. Incubate the culture at 37°C in a humidified 5% CO2/95% air incubator. Perform a 100% medium change every day, passage cells every 1-2 days.
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 10% DMSO
Culture Conditions
Temperature: 37°C
Growth conditions: Use a feeder layer, LIF and frequent subcultures to prolong the undifferentiated state
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor TJ Ley
Year of Origin 1998
References

Hughes, DE et al. Genetic variation in C57BL/6 ES cell line. Mamm. Genome 18: 549-558, 2007. PubMed: 17828574

Ware, BC, et al. Utiltiy of a C57BL/6 ES line versus 129 ES lines for targeted mutations in mice. Transgenic Res. 12: 743-746, 2003. PubMed: 14713204

Graubert A T, et al. Stochastic, stage-specific mechanisms account for the variegation of a human globin transgene. Nucleic Acids Research. 26 (12) :2849-2858, 1998. PubMed: 9611227

Basic Documentation
Restrictions

Prior to purchase, for-profit commercial institutions must obtain a license agreement. For instructions on how to proceed, please contact ATCC's Office of Licensing and Business Development at licensing@atcc.org or 703 365 2773.

References

Hughes, DE et al. Genetic variation in C57BL/6 ES cell line. Mamm. Genome 18: 549-558, 2007. PubMed: 17828574

Ware, BC, et al. Utiltiy of a C57BL/6 ES line versus 129 ES lines for targeted mutations in mice. Transgenic Res. 12: 743-746, 2003. PubMed: 14713204

Graubert A T, et al. Stochastic, stage-specific mechanisms account for the variegation of a human globin transgene. Nucleic Acids Research. 26 (12) :2849-2858, 1998. PubMed: 9611227