5E9C11 (ATCC® HB-21)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia
Applications
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-22.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-22.
Genes Expressed
immunoglobulin; monoclonal antibody; against human T cell (human T lymphocyte); also reacts with human transferrin receptor
Tumorigenic Yes
Effects
Yes, in pristane primed BALB/c mice
Comments
Animals were immunized with HSB-2 cells (ATCC CCL-120.1, an acute lymphocytic T leukemia cell line).
Spleen cells were fused with P3X63Ag8 myeloma cells.
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-22.
The antibody recognizes a 90000 dalton cell surface antigen (the same as that recognized by OKT 9, ATCC CRL-8021).
The antibody precipitates human transferrin receptor.
It does not fix complement.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
This line has carried by passage as ascites in pristane primed (0.5 ml, 7 days prior to injection of cells) mice.
The mice are injected with 0.3 to 0.5 ml of cells, and the ascites is collected 7 to 10 days later.
Recent information from the donor indicates that the cells are readily grown in culture using the above medium.
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 5 X 10 exp4 cells/ml and do not allow the cells to exceed 8 X 10 exp5 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor A Fauci, BF Haynes
References

Haynes BF, et al. Characterization of a monoclonal antibody (5E9) that defines a human cell surface antigen of cell activation. J. Immunol. 127: 347-351, 1981. PubMed: 6787129

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Haynes BF, et al. Characterization of a monoclonal antibody (5E9) that defines a human cell surface antigen of cell activation. J. Immunol. 127: 347-351, 1981. PubMed: 6787129