Certified Reference Materials (CRMs)

• Mycoplasma hyorhinis (ATCC^® qCRM-17981D^™)

  ATCC^® Number: qCRM-17981D^™
  

  Strain Designations: Genomic DNA from Mycoplasma hyorhinis strain BTS-7 [ATCC 23234, D.G. ff. Edward PG 42, NCTC 10130]

  Type Strain: yes

  Biosafety Level: 1

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  Biosafety Level:	1
  Product Format:	frozen Specification range: 1x106 - 1x107 genome copies/µL

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• NCI-H441 [H441] (ATCC^® CRM-HTB-174^™)

  ATCC^® Number: CRM-HTB-174^™
  

  Organism: Homo sapiens, human

  Cell Type: Epithelial Cell (Kras Crm)

  Tissue: Lung

  Disease Papillary Adenocarcinoma

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  Tissue:	Lung
  Product Format:	frozen

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• Bacillus subtilis subsp. spizizenii Nakamura et al. (ATCC^® CRM-6633^™)

  ATCC^® Number: CRM-6633^™
  

  Deposited As Bacillus subtilis (Ehrenberg) Cohn

  Strain Designations: NRS 231

  Type Strain: no

  Biosafety Level: 1

  For-Profit:   $420.00 Non-Profit:   $357.00

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  Biosafety Level:	1
  Product Format:	freeze-dried

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• High molecular weight genomic DNA isolated from A-549 Lung Carcinoma cell line. (ATCC^® CRM-CCL-185D^™)

  ATCC^® Number: CRM-CCL-185D^™
  

  Quantity: 5 μg

  Storage: -80°C or colder

  Applications: For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

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  Product Format:

  5 μg

  Comments:

  Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed. Certified Reference Material produced under an ISO Guide 34:2009 accredited process. This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>A (correlates to protein sequence p.G12S).

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• SRM Genomic DNA Standards for HER2 Measurements (ATCC^® NIST-2373^™)

  ATCC^® Number: NIST-2373^™
  

  Quantity: 100 µL of DNA solution per vial

  Applications: Used to assign ratios of the human epidermal growth factor 2 gene (HER2; the official gene symbol ERBB2) to unamplified reference genes. Measurements of the amplification (increased copies) of the HER2 gene in breast cancer samples are used as a biomarker for determining the classification for breast cancers

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  Product Format:

  100 µL of DNA solution per vial

  Comments:

  SRM NIST-2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the HER2 gene. The five purified genomic DNAs were solubilized in a buffer consisting of 10 mmol/L tris(hydroxymethyl)aminomethane and 0.1 mmol/L ethylenediaminetetraacetic acid disodium salt (EDTA) pH 8.0 (TE–4). The five components are genomic DNA materials derived from human cell lines SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474, labeled A, B, C, D, and E, respectively. A unit of SRM 2373 consists of five vials, one of each component, containing approximately 100 μL of DNA solution. Each of these vials is labeled and is sealed with a color-coded screw cap. See the NIST Certificate of Analysis for directions for use and quality control specifications.  Certificate of Analysis NIST SDS

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• High molecular weight genomic DNA isolated from MIA PaCa-2; Pancreatic Carcinoma; Human cell line. (ATCC^® CRM-CRL-1420D^™)

  ATCC^® Number: CRM-CRL-1420D^™
  

  Quantity: 5 μg

  Storage: -80°C or colder

  Applications: For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

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  Product Format:

  frozen 5 μg

  Comments:

  Store at   - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed. Certified Reference Material produced under an ISO Guide 34:2009 accredited process. This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>T (correlates to protein sequence p.G12C).

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• Clostridium sporogenes (Metchnikoff) Bergey et al. (ATCC^® CRM-11437^™)

  ATCC^® Number: CRM-11437^™
  

  Deposited As Clostridium sporogenes (Metchnikoff) Bergey et al.

  Strain Designations: L.S. McClung 2006

  Type Strain: no

  Biosafety Level: 1

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  Biosafety Level:	1
  Product Format:	freeze-dried

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• Aspergillus brasiliensis Varga et al. (ATCC^® CRM-16404^™)

  ATCC^® Number: CRM-16404^™
  

  Deposited As Aspergillus niger van Tieghem, anamorph

  Designation: WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275]

  Product Format: freeze-dried

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  Comments:	Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request. Certified Reference Material produced under an ISO Guide 34:2009 accredited process. This strain was recently re-named as Aspergillus brasiliensis according to the reference Varga et al. 2007.  Use of impedance for preservative efficacy testing
  Type Strain:	no
  Biological Safety Level:	1

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• SRM PCR-Based DNA Profiling Standard (ATCC^® NIST-2391c^™)

  ATCC^® Number: NIST-2391c^™
  

  Storage: Store in the dark between 2°C to 8°C. DO NOT FREEZE.

  Applications: For use in the standardization of forensic and paternity quality assurance procedures for polymerase chain reaction (PCR)-based genetic testing, for instructional law enforcement or non-clinical research purposes, and for quality assurance when assigning values to in-house control materials. It is not intended for any human or animal clinical diagnostic use.

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  Comments:

  SRM NIST-2391c is composed of well-characterized human deoxyribonucleic acid (DNA) in two forms: genomic DNA (Components A through D) and DNA to be extracted from cells that have been spotted onto 903 paper (Component E) and FTA paper (Component F).   Component Description Amount Concentration(a) A Anonymous single-source female genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL B Anonymous single-source male genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL C Anonymous single-source male genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL D Mixed-source (Components A and C) genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL E Anonymous single-source female cells spotted on 903 paper Two 6 mm punches 7.5 × 104 cells per punch F Anonymous single-source male cells spotted on FTA paper Two 6 mm punches 7.5 × 104 cells per punch   See the NIST Certificate of Analysis for directions for use and quality control specifications.  Certificate of Analysis NIST SDS

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• Candida albicans (Robin) Berkhout (ATCC^® CRM-10231^™)

  ATCC^® Number: CRM-10231^™
  

  Deposited As Candida albicans (Robin) Berkhout, anamorph

  Alternate State: Monilia pinoyi (Castellani) Castellani et Chalmers; Endomyces albicans (Robin) Vuillemin; Candida claussenii Lodder et Kreger-van Rij, anamorph; Candida stellatoidea (Jones et Martin) Langeron et Guerra, anamorph

  Designation: 3147 [CBS 6431, CCY 29-3-106, CIP 48.72, DSM 1386, IFO 1594, NCPF 3179, NCYC 1363, NIH 3147, VTT C-85161]

  Product Format: freeze-dried

  For-Profit:   $420.00 Non-Profit:   $357.00

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  Comments:	Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request. Certified Reference Material produced under an ISO Guide 34:2009 accredited process. For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. For sterility testing, not more than five passages from the ATCC culture should be used.
  Type Strain:	no
  Biological Safety Level:	1

  • 
    Product Sheet
  • Certificate
    of Analysis
  • SDS

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