• remove Product Category: Cell Lines & Hybridomas

Certified Reference Materials (CRMs)

  • High molecular weight genomic DNA isolated from HuT 78; Cutaneous T Cell Lymphoma; Human cell line. (ATCC® CRM-TIB-161D)

    ATCC® Number: CRM-TIB-161D

    Quantity: 5 μg
    Storage: -80°C or colder
    Applications:

    For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

    Preview Product

    Product Format: frozen 5 μg
    Comments:

    Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: None (wild-type).

  • SRM Human DNA Quantitation Standard (ATCC® NIST-2372)

    ATCC® Number: NIST-2372

    Quantity: 110 µL of DNA solution per vial
    Storage: Store in the dark between 2°C to 8°C. Do not freeze
    Applications: forensic quantitation

    For-Profit:   $811.00 Non-Profit:   $811.00

    Preview Product

    Product Format: 110 µL of DNA solution per vial
    Comments: SRM NIST-2372 is intended primarily for use in the value assignment of human genomic DN forensic quantitation materials. It is not intended for any human or animal clinical diagnostic use. SRM 2372 consists of three well-characterized human genomic DNA materials solubilized in 10 mmol/L 2-amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride (Tris HCl) and 0.1 mmol/L ethylenediaminetetraacetic acid disodium salt (disodium EDTA) using deionized water adjusted to pH 8.0 buffer (TE-4). The three component genomic DNA materials, labeled A, B, and C, are derived from a single male donor, multiple female donors, and multiple male and female donors, respectively. A unit of the SRM consists of one sterile 2 mL vial of each component, each vial containing approximately 110 μL of DNA solution. Each of these vials is labeled and is sealed with a color–coded screw cap.

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS


  • High molecular weight genomic DNA isolated from MIA PaCa-2; Pancreatic Carcinoma; Human cell line. (ATCC® CRM-CRL-1420D)

    ATCC® Number: CRM-CRL-1420D

    Quantity: 5 μg
    Storage: -80°C or colder
    Applications: For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

    Preview Product

    Product Format: frozen 5 μg
    Comments:

    Store at   - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>T (correlates to protein sequence p.G12C).


  • High molecular weight genomic DNA isolated from A-549 Lung Carcinoma cell line. (ATCC® CRM-CCL-185D)

    ATCC® Number: CRM-CCL-185D

    Quantity: 5 μg
    Storage: -80°C or colder
    Applications: For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

    Preview Product

    Product Format: 5 μg
    Comments:

    Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>A (correlates to protein sequence p.G12S).

  • SRM Genomic DNA Standards for HER2 Measurements (ATCC® NIST-2373)

    ATCC® Number: NIST-2373

    Quantity: 100 µL of DNA solution per vial
    Applications: Used to assign ratios of the human epidermal growth factor 2 gene (HER2; the official gene symbol ERBB2) to unamplified reference genes. Measurements of the amplification (increased copies) of the HER2 gene in breast cancer samples are used as a biomarker for determining the classification for breast cancers

    For-Profit:   $1,473.00 Non-Profit:   $1,473.00

    Preview Product

    Product Format: 100 µL of DNA solution per vial
    Comments: SRM NIST-2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the HER2 gene. The five purified genomic DNAs were solubilized in a buffer consisting of 10 mmol/L tris(hydroxymethyl)aminomethane and 0.1 mmol/L ethylenediaminetetraacetic acid disodium salt (EDTA) pH 8.0 (TE–4). The five components are genomic DNA materials derived from human cell lines SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474, labeled A, B, C, D, and E, respectively. A unit of SRM 2373 consists of five vials, one of each component, containing approximately 100 μL of DNA solution. Each of these vials is labeled and is sealed with a color-coded screw cap.

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS

  • Human DNA for Whole-Genome Variant Assessment (Daughter of Utah/European Ancestry) (ATCC® NIST-8398)

    ATCC® Number: NIST-8398

    Storage: NIST-8398 is stored at −20°C for long-term storage, or in the dark at 4°C for short-term storage (if use is imminent). Please limit freeze-thaw cycles
    Applications: This Reference Material (RM) is intended for assessing performance of human genome sequencing, including whole genome sequencing, whole exome sequencing, and more targeted sequencing such as gene panels. Specifically, the material can be used to obtain estimates of true positives, false positives, true negatives, and false negatives for variant calls. This genomic DNA is to be analyzed as any other processed, extracted DNA. It can be used to assess sequencing library preparation, sequencing machines, and the bioinformatics steps of mapping, alignment, and variant calling. This RM is not intended to assess subsequent bioinformatics steps such as functional or clinical interpretation. 

    This RM is intended to provide a whole human genome sample and accompanying reference values to assess performance of variant calling from genome sequencing

    For-Profit:   $608.00 Non-Profit:   $608.00

    Preview Product

    Comments: This Reference Material (RM) is intended to provide a whole human genome sample and accompanying reference values to assess performance of variant calling from genome sequencing. This RM contains human genomic deoxyribonucleic acid (DNA) extracted from a large growth of the human lymphoblastoid cell line GM12878. A unit of RM 8398 consists of a single vial containing approximately 10 μg of genomic DNA, with the peak of the nominal length distribution longer than 48.5 kb, as referenced by Lambda DNA, and the DNA is in TE buffer (10 mM TRIS, pH 8.0, 1 mM EDTA, pH 8.0).

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS


  • High molecular weight genomic DNA isolated from RPMI 8226; Plasmacytoma; Human cell line. (ATCC® CRM-CCL-155D)

    ATCC® Number: CRM-CCL-155D

    Quantity: 5 μg
    Storage: -80°C or colder
    Applications:

    For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

    Preview Product

    Product Format: 5 μg
    Comments:

    Store at   - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Heterozygous DNA Change: c.35G>C (correlates to protein sequence p.G12A)

  • High molecular weight genomic DNA isolated from PSN-1; Pancreas Adenocarcinoma; Human cell line. (ATCC® CRM-CRL-3211D)

    ATCC® Number: CRM-CRL-3211D

    Quantity: 5 μg
    Storage: -80°C or colder
    Applications: For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

    Preview Product

    Product Format: frozen 5 μg
    Comments:

    Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>C (correlates to protein sequence p.G12R).


  • SRM PCR-Based DNA Profiling Standard (ATCC® NIST-2391c)

    ATCC® Number: NIST-2391c

    Storage: Store in the dark between 2°C to 8°C. DO NOT FREEZE.
    Applications: For use in the standardization of forensic and paternity quality assurance procedures for polymerase chain reaction (PCR)-based genetic testing, for instructional law enforcement or non-clinical research purposes, and for quality assurance when assigning values to in-house control materials. It is not intended for any human or animal clinical diagnostic use.

    For-Profit:   $1,119.00 Non-Profit:   $1,119.00

    Preview Product

    Comments: SRM NIST-2391c is composed of well-characterized human deoxyribonucleic acid (DNA) in two forms: genomic DNA (Components A through D) and DNA to be extracted from cells that have been spotted onto 903 paper (Component E) and FTA paper (Component F).

     

    Component Description Amount Concentration(a)
    A Anonymous single-source female genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    B Anonymous single-source male genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    C Anonymous single-source male genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    D Mixed-source (Components A and C) genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    E Anonymous single-source female cells spotted on 903 paper Two 6 mm punches 7.5 × 104 cells per punch
    F Anonymous single-source male cells spotted on FTA paper Two 6 mm punches 7.5 × 104 cells per punch

     

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS

     

  • High molecular weight genomic DNA isolated from CCRF-CEM; Leukemia; Human cell line. (ATCC® CRM-CCL-119D)

    ATCC® Number: CRM-CCL-119D

    Quantity: 5 μg
    Storage: - 80°C or colder
    Applications:

    For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

    Preview Product

    Product Format: frozen 5 μg
    Comments:

    Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Heterozygous DNA Change c.35G>A (correlates to protein sequence p.G12D)