Human poliovirus 1 (ATCC® VR-59)

Classification: Picornaviridae, Enterovirus, Enterovirus C  /  Product Format: freeze-dried

Classification Picornaviridae, Enterovirus, Enterovirus C
Deposited As Poliovirus 1
Agent Human poliovirus 1
Strain LSa
Applications
testing
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
LS-a virus is a variant or mutant isolated from the Mahoney strain. (The Mahoney strain was isolated by T. Francis in 1941 from a pool of 3 stools from asymptomatic cases of poliomyelitis in Cleveland.)
Product Format freeze-dried
Storage Conditions -70°C or colder
Comments
[The BMBL notes that all laboratory personnel working directly with the agent must have documented polio vaccination or demonstrated serologic evidence of immunity to all three poliovirus types. (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4)].
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, rounded and sloughing cells in culture.
Yes, in vivo effects: paralysis in mouse
Recommended Host
mouse, i. s. inoculation; LLC-MK2 cells (ATCC CCL-7)

Production Host: LLC-MK2 (ATCC® CCL-7)

mouse; monkey, i. s. inoculation; monkey kidney cells
Growth Conditions
Duration: 2-10 days
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, rounded and sloughing cells in culture.
Yes, in vivo effects: paralysis in mouse
Name of Depositor M Schaeffer
Source
LS-a virus is a variant or mutant isolated from the Mahoney strain. (The Mahoney strain was isolated by T. Francis in 1941 from a pool of 3 stools from asymptomatic cases of poliomyelitis in Cleveland.)
References

Li CP, Schaeffer M. Adaptation of type I poliomyelitis virus to mice. Proc. Soc. Exp. Biol. Med. 82: 477-481, 1953. PubMed: 13047436

Li CP, et al. Experimentally produced variants of poliomyelitis virus combining in vivo and in vitro techniques. Ann. N.Y. Acad. Sci. 61: 902-910, 1955. PubMed: 13340599

Water supply -- Domestic type water treatment appliances -- Performance requirements. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4348:1995.

Cross References

Nucleotide (GenBank) : AJ295187 Human poliovirus 1 genomic RNA for partial polyprotein gene, isolate VR-59, ATCC.