Candida albicans (Robin) Berkhout

Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO 17034 accredited process.

For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.

For sterility testing, not more than five passages from the ATCC culture should be used.

1. Open an ampoule according to enclosed instructions.
2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1­-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

Colonies are white to cream-colored, butyrous, with entire margins.  Cells globose-ovoid-ellipsoid, reproducing by budding, usually also generating straight or branced chains of cells (sometimes elongate) ["pseudohyphae"].  On some media producing true septate hyphae and thick-walled globose-obovoid chlamydospores.

Certified Reference Material produced under an ISO 17034 accredited process.

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