pDC47 (ATCC® 87028)

Applications: YI-type (integrating) shuttle vector  /  Depositors: RH Reeves

Permits and Restrictions

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Designations pDC47
Depositors RH Reeves
Biosafety Level 1
Vector Information
Size (kb): 6.0999999046325680
Vector: pDC47 (plasmid)
Construction: pBP47, pol2sneo
Marker(s):G418R,ampR,HIS3
Construct size (kb): 6.099999904632568
Features: marker(s): ampR, HIS3, G418R
replicon: pMB1
terminator: SV40
Applications
YI-type (integrating) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.8, 0.3; PvuI--6.1; EcoRI--4.0, 2.3.
Linearizing with SmaI and transformating into a YAC-containing his3 deletion strain will target integration to Alu sequences. Linearizating at the PvuI site will target integration to the TRP1 centric arm of pYAC4 derivatives.
His+ transformants can then be transferred to a eukaryotic cell by spheroplast fusion.
YI-type shuttle integrative vector useful for incorporating a neoR cassette into YACs.
Constructed by ligating the 1.35 kb XhoI fragment of pPOL2shortneobpA to the largest PvuII fragment of pBP47.
The order of the major features in this plasmid is: pMB1 ori - bla - HIS3 - BamHI - SmaI - BamHI - pol2sneo.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Pavan WJ, et al. Modification and transfer into an embryonal carcinoma cell line of a 360-kilobase human-derived yeast artificial chromosome. Mol. Cell. Biol. 10: 4163-4169, 1990. PubMed: 2196449

Reeves RH, et al Introduction of YACs into mammalian cells. :;Current protocols in human genetics submitted, 1994

Roger H Reeves, personal communication

Shipped freeze-dried
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