Pseudomonas aeruginosa (Schroeter) Migula

15442 ^™

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Pseudomonas aeruginosa strain PRD-10 [CIP 103467, NCIB 10421, PCI 812] is a whole genome-sequenced strain that was isolated from a water bottle in an animal room. This bacterium can be used in disinfectant testing, antimicrobial preservative assays, testing of antimicrobial handwashing formulations, testing of slimicide, and quality control of Biosynth products. It is recommended by ATCC for use in the test described in ASTM Standard Test Method E686-91 where only the taxon is specified.

Product category: Bacteria
Strain designation: PRD-10 [CIP 103467, NCIB 10421, PCI 812]
Type strain: No
Genome sequenced strain: Yes
Isolation source: Water bottle in animal room
Applications: Bioinformatics

Food testing

Quality control

Pharmaceutical testing
Product format: Freeze-dried
Storage conditions: 2°C to 8°C

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Documentation

Product sheet

BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Specific applications

Assay of antimicrobial preservatives

Bacterial resistance testing textiles

Efficacy testing

Quality control strain

Testing

Testing antimicrobial agent

Testing antimicrobial handwashing formulations

Testing disinfectants

Testing slimicide

Testing bactericides

Quality control strain for Biosynth products

Food testing

Opportunistic pathogen research

Pharmaceutical and Personal Care

Recommended as a test microorganism for USP <1072> Disinfectants and Antiseptics

Recommended as a test microorganism for EPA Method MB-05-16: AOAC Use Dilution Method for Testing Disinfectants

Recommended as a test microorganism for EPA Method MB-06-10: Germicidal Spray Products as Disinfectants (GSPT): Testing of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica

Recommended as a test microorganism for EPA Method MB-09-08: Disinfectant Towelette Test: Testing of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica

Recommended as a test microorganism for EPA Method MB-19-06: Preparing a Pseudomonas aeruginosa or Staphylococcus aureus Biofilm using the CDC Biofilm Reactor

Recommended as a test microorganism for EPA Method MB-25-05: OECD Quantitative Method for Evaluating Bactericidal and Mycobactericidal Activity of Microbicides Used on Hard, Non-Porous Surfaces

Recommended as a test microorganism for EPA Method MB-41-00: Method for the Evaluation of Antimicrobial Activity of Hard, Nonporous Copper-Containing Surface Products

Recommended as a test microorganism for AENOR UNE-EN 1276: Evaluation of chemical disinfectant or antiseptic for bactericidal activity

Recommended as a test microorganism for ASTM E2562: Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with High Shear and Continuous Flow using CDC Biofilm Reactor

Preceptrol

Characteristics

Comments: This strain is recommended by ATCC for use in the test described in ASTM Standard Test Method E686-91 where only the taxon is specified.
Broad spectrum of resistance to various commercial germicides
Pyocyanin not produced

Handling information

Medium

ATCC Medium 18: Trypticase Soy Agar/Broth

Temperature

37°C

Atmosphere

Aerobic

Handling procedure

Open vial according to enclosed instructions.
Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
Incubate the tubes and plate at 37°C for 24 hours.

Handling notes

Two colony types may be observed on maintenance media.
Purified genomic DNA of this strain is available as ATCC 15442D-5.
Additional information on this culture is available on the ATCC web site at www.atcc.org.

Quality control specifications

Verification method: Whole-genome Sequencing

History

Deposited as: Pseudomonas aeruginosa (Schroeter) Migula
Depositors: A Beloian
Chain of custody: ATCC <-- A Beloian <-- L Herman
Cross references: GenBank Z22989 P. aeruginosa (ATCC 15442) gene for 16S ribosomal RNA (partial).

GenBank AF094718 Pseudomonas aeruginosa strain ATCC 15442 16S ribosomal RNA gene, partial sequence.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

AOAC International Testing disinfectants against Pseudomonas aeruginosa, phenol coefficient method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 955.13.

AOAC International Testing disinfectants against Pseudomonas aeruginosa, hard surface carrier test method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 991.49.

US General Services Administration Disinfectant, general purpose (liquid phenolic type). Washington, DC:US General Services Administration;Commercial Item Description A-A-1438A, 1997

US General Services Administration Disinfectant-detergent, general purpose (phenolic type). Washington, DC:US General Services Administration;Commercial Item Description A-A-1439A, 1998

US General Services Administration Disinfectant-detergent, general purpose (iodophor). Washington, DC:US General Services Administration;Commercial Item Description A-A-1440, 1981

View All Curated Citations for this Product

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