NIST Nucleic Acids

  • Human DNA for Whole-Genome Variant Assessment (Daughter of Utah/European Ancestry) (ATCC® NIST-8398)

    ATCC® Number: NIST-8398

    Storage: NIST-8398 is stored at −20°C for long-term storage, or in the dark at 4°C for short-term storage (if use is imminent). Please limit freeze-thaw cycles
    Applications: This Reference Material (RM) is intended for assessing performance of human genome sequencing, including whole genome sequencing, whole exome sequencing, and more targeted sequencing such as gene panels. Specifically, the material can be used to obtain estimates of true positives, false positives, true negatives, and false negatives for variant calls. This genomic DNA is to be analyzed as any other processed, extracted DNA. It can be used to assess sequencing library preparation, sequencing machines, and the bioinformatics steps of mapping, alignment, and variant calling. This RM is not intended to assess subsequent bioinformatics steps such as functional or clinical interpretation. 

    This RM is intended to provide a whole human genome sample and accompanying reference values to assess performance of variant calling from genome sequencing

    For-Profit:   $608.00 Non-Profit:   $608.00

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    Comments: This Reference Material (RM) is intended to provide a whole human genome sample and accompanying reference values to assess performance of variant calling from genome sequencing. This RM contains human genomic deoxyribonucleic acid (DNA) extracted from a large growth of the human lymphoblastoid cell line GM12878. A unit of RM 8398 consists of a single vial containing approximately 10 μg of genomic DNA, with the peak of the nominal length distribution longer than 48.5 kb, as referenced by Lambda DNA, and the DNA is in TE buffer (10 mM TRIS, pH 8.0, 1 mM EDTA, pH 8.0).

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS


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