Caviid herpesvirus 2 (ATCC® VR-682)

Classification: Herpesviridae, Unassigned  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Herpesviridae, Unassigned
Agent Caviid herpesvirus 2
Strain 22122
Biosafety Level 2
Isolation
Submaxillary glands from a guinea pig
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Guinea pig lung (ATCC® CCL-158™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 9-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% or greater of the monolayer.
Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor MJ Collins, Jr., Microbiological Associates, Inc.
Source Submaxillary glands from a guinea pig
References

Hartley JW, et al. Serial propagation of the guinea pig salivary gland virus in tissue culture. Proc. Soc. Exp. Biol. Med. 96: 281-285, 1957. PubMed: 13485080

Cross References

Nucleotide (GenBank) : AB592928.1 Caviid herpesvirus 2 DNA, complete genome, strain: 22122

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation