Human echovirus 2 (ATCC® VR-1867)

Classification: Picornaviridae, Enterovirus, Human enterovirus B  /  Product Format: frozen

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Classification Picornaviridae, Enterovirus, Human enterovirus B
Deposited As Echovirus 2
Agent Human echovirus 2
Strain Cornelis
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
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Comments Derived by adaptation of primary cell culture product ATCC® VR-1039™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1867™ and ATCC® VR-1039™ have not been compared for sequence or infectivity in primary cell culture and tissue culture.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Vero cells (ATCC® CCL-81™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 16-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 4-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor NIH/NIAID
Source Feces from a patient with aseptic meningitis, Connecticut, 1951.
References

Melnick J. Application of tissue culture methods to epidemiological studies of poliomyelitis. Am. J. Public Health 44: 571-580, 1954. PubMed: 13148397

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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