Nitrosopumilus maritimus (TSD-97)

Strain Designations: SCM1  /  Biosafety Level: 1

Permits And Restrictions

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Strain Designations SCM1
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Depositor Recommended Growth Conditions

Bicarbonate-buffered synthetic crenarchaeota medium aerobically in the dark at 25°C with no shaking for 10 days to reach stationary phase.

Bicarbonate-buffered synthetic crenarchaeota medium (per liter)
26 g NaCl
5 g MgSO4·7H2O
5 g MgCl2·6H2O
1.5 g CaCl2·2H2O
0.1 g KBr
3 ml NaHCO3 (1 M)
5 ml KH2PO4 (0.4 g/l)
1 ml FeNaEDTA solution (7.5 M)
1 ml trace element solution
0.2 ml NH4Cl (1 M)
0.1 ml alpha-ketoglutaric acid (100 mM).

Trace element solution (per liter)
8 ml conc. HCl (~12.5M)
30 mg H3BO3
100 mg MnCl2·4H2O
190 mg CoCl2·6H2O
24 mg NiCl2·6H2O
2 mg CuCl2·2H2O
144 mg ZnSO4·7H2O
36 mg Na2MoO4·2H2O
Name of Depositor DA Stahl, University of Washington, Seattle, Washington, USA.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation