PhlF Activation Pair (TetR Homolog) (ATCC® SB-2005)

Permits and Restrictions

View Permits

Preparation
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Components
Synthetic activator:
pENTR_L1L2_PhlF -VP64 (ATCC SB-1099)

Synthetic promoter:
pENTR_L4R1_PhlF -pCYC1MIN (ATCC SB-1060)
Application A plasmid pair used to construct a gene expression system via a TetR homolog-based activation system
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The synthetic activator is a fusion of the TetR homolog PhlF and a VP64 activation domain.
Product Format dried purified plasmid DNA (2 to 3 µg/vial)
Storage Conditions 4°C or colder
Depositor R Weiss, Massachusetts Institute of Technology
References

Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737

Ron Weiss, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737

Ron Weiss, personal communication