Primary CD56+ NK Cells (ATCC® PCS-800-019)

Organism: Homo sapiens, human  /  Tissue: blood  / 

Permits and Restrictions

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Organism Homo sapiens, human
Tissue blood
Morphology lymphoid
Growth Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age lot-specific
Gender lot-specific
Ethnicity lot-specific
Applications Research purposes, compound screening, immune function assays such as the Natural Killer Cell Assay.

See Application Note No. 31: A simple and rapid alternative to 51chromium or fluorescent dye loading for quantification of natural killer cell activity: ATCC K-562-GFP cells for more information.

Product Format frozen 1.0 mL
Images PCS-800-019 Application Data
Comments CD56+ Natural killer (NK) cells are the major effectors of the innate immune system and are important in host defense, cancer, and autoimmunity.
CD56+ Natural Killer Cells are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures.
ATCC CD56+ Natural Killer cells are tested by flow cytometry for the following surface markers:  
  • CD45+: ≥ 90% 
  • CD56+: ≥ 90% 
  • CD56+CD3-: Report results 
  • CD56+CD16+: Report results
Complete Growth Medium Natural Killer Cells have a limited lifespan in culture and should be thawed immediately and only prior to their intended use in application specific media. ATCC recommends thawing them in RPMI-1640 (ATCC 30-2001). ATCC does not recommend maintaining Natural Killer Cells in culture in the absence of application-specific growth factors.
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Volume 1.0 mL
Cells per Vial ≥ 5 x 106
Viral Testing Hepatitis B: Negative
Hepatitis C: Negative
HIV: Negative
HTLV-I/II: Negative
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQs
  1. Human primary immune cells per vial PCS-800-019

    Each ATCC vial contains at least 5x106 viable cells.


    Date Updated: 2/26/2018
  2. Cryopreservative used with Primary Immune cells PCS-800-019

    The cells are cryopreserved in a serum‐free and xeno‐free medium (CryoStor® CS10 Freeze Medium from BioLife Solutions). 


    Date Updated: 2/26/2018
  3. Cell surface marker expression of ATCC Human Primary Immune cells PCS-800-019

    Each lot is tested for expression of CD56, CD45, CD3 and CD16. Each lot is tested to ensure


    Date Updated: 2/26/2018
  4. Human primary NK cell expansion PCS-800-019

    Yes, with proper stimuli (typically via cytokines and/or under co‐culture conditions) natural killer


    Date Updated: 2/26/2018
  5. Cytotoxicity assays PCS-800-019

    Yes, these cells will exhibit cytotoxic activity in vitro against classical natural killer cell targets.


    Date Updated: 2/26/2018
  6. Post-thaw yield and viability of Primary CD56+ NK Cells (ATCC® PCS-800-019™)

    We recommend the following thawing procedure:


    Date Updated: 2/26/2018