CD4 Helper T Cells oversee the action of the human immune system; they are involved in activating and directing other immune cells. CD4+ Helper T Cells are crucial in (1) determining B cell antibody class switching, (2) the activation and growth of Cytotoxic T Cells, and (3) maximizing bactericidal activity of phagocytes (i.e., macrophages).
CD4+ Helper T Cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.
CD4+ Helper T Cells are tested by flow cytometry for the following surface markers:
- CD3+: ≥ 90%
- CD4+: ≥ 90%
- CD45+: ≥ 90%