||The kit contains components that when added to the Mesenchymal Stem Cell Basal Media (ATCC PCS-500-030) create a complete ATCC® Primary Cell Solutions™ culture environment for muscle cells derived from normal skeletal muscle tissue. The medium formulation is designed to support normal skeletal cell proliferation and growth. No feeder layers, extracellular matrix proteins or other substrates re required.
The final concentration for each component in complete skeletal muscle cell growth medium is as follows:
- L-Glutamine: 10nm
- rh EGF: 5 ng/mL
- Dexamethasone: 5mM
- rh FGF-b: 5 ng/mL
- rh Insulin: 25 µg/mL
- Stem Cell Qualified FBS: 4%
Note: The Skeletal Cell Differentiation Tool (PCS-950-050) is needed to fully differentiate Skeletal Muscle Cells.