• remove Product Category: Primary Cells

Specialty Cell Culture Media

  • (ATCC® )

    ATCC® Number: ACS-4007

    Storage: -20°C
    Applications: Add to DMEM:F12 (ATCC® 30-2006™) to produce complete medium for hTERT Immortalized RPTEC cells (ATCC® CRL-4031™)

    For-Profit:   $0.00 Non-Profit:   $0.00

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    Comments: Each growth kit contains:
    PCS-999-058 5 ml RPTEC Supplement A
    PCS-999-059 10 ml RPTEC Supplement B

    Required but not supplied: G418 solution MUST be added to the above medium to a final concentration of 0.1 mg/mL G418 to maintain the selective pressure for immortalization 

    The hTERT immortalized RPTEC Growth Kit contains components that when added to DMEM:F12 (ATCC® 30-2006™) create a complete cell culture environment for hTERT immortalized RPTEC cells (ATCC® CRL-4031™). The final concentration for each growth kit component in the complete hTERT immortalized RPTEC growth medium is as follows:
    25 ng/mL Hydrocortisone
    3.5 µg/mL Ascorbic Acid
    8.65 ng/mL Sodium selenite
    5.0 µg/mL Transferrin
    5.0 µg/mL insulin
    5 pM Triiodo-L-thyronine
    25 ng/mL Prostaglandin E1
    10 ng/mL rhEGF
    1.2 µg/mL Sodium Bicarbonate 

    Note: Fetal Bovine Serum is not required for proliferation but may be added if it is desired to transfect the cells (final FBS concentration in complete medium is 0.2%).

  • siFEX RNAi Transfection Reagent (ATCC® ACS-4006)

    ATCC® Number: ACS-4006

    Quantity: 500 µL
    Storage: Store at 2°C to 8°C
    Applications: Transient transfection, stable transfection, gene expression, siRNA transfection, miRNA transfection, gene knockdown.

    For-Profit:   $265.00 Non-Profit:   $265.00

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    Product Format: 500 µL
    Comments:

    siFEX RNAi Transfection Reagent is designed for transfection of small RNA sequences including siRNA and miRNA precursors into a broad array of mammalian cells with high efficiency and minimal toxicity. Cell line-specific protocols can be found here.

  • TransfeX™ Transfection Reagent (ATCC® ACS-4005)

    ATCC® Number: ACS-4005

    Quantity: 500 μL
    Storage: Store at 2°C to 8°C
    Applications: Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.

    For-Profit:   $230.00 Non-Profit:   $230.00

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    Product Format: 500 μL
    Comments:

    Reagent is designed for transfection of primary cells and hard-to-transfect cell lines.  TransfeX Transfection Reagent provides high efficiency with low cell mortality. Cell line-specific protocols can be found here.

  • For-Profit:   $78.00 Non-Profit:   $78.00

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  • Mammary Epithelial Cell Basal Medium (ATCC® PCS-600-030)

    ATCC® Number: PCS-600-030

    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040).

    ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $82.00 Non-Profit:   $82.00

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  • GeneXPlus Transfection Reagent (ATCC® ACS-4004)

    ATCC® Number: ACS-4004

    Quantity: 1 mL
    Storage: -20°C
    Applications: Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.  Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266). A complete list of cell line-specific protocols can be found here.

    For-Profit:   $357.00 Non-Profit:   $357.00

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    Product Format: 1 mL
    Comments:

    This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.

     

    GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

  • Dermal Cell Basal Medium (ATCC® PCS-200-030)

    ATCC® Number: PCS-200-030

    Quantity: 485 ml
    Storage: 2 to 8°C, protect from light
    Applications: Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions™ system.

    This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. 

    To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCC PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCC PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation.

    To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCC PCS-200-041) or the Adult Melanocyte Growth Kit (ATCC PCS-200-042).

    Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

     

    For-Profit:   $51.00 Non-Profit:   $51.00

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  • For-Profit:   $26.50 Non-Profit:   $26.50

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  • For-Profit:   $52.00 Non-Profit:   $52.00

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  • For-Profit:   $101.00 Non-Profit:   $101.00

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