MCDB 131 Medium without L-Glutamine (ATCC® 30-2603)

Permits and Restrictions

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Product Format 500 mL
Storage Conditions Store medium at 2°C to 8°C in the dark when not in use.

MCDB 131 Medium provides a defined and nutritionally balanced environment for mammalian cell culture. The medium includes trace elements and higher levels of certain vitamins and amino acids compared to basal medium. MCDB 131 Medium was originally developed for human microvascular endothelial cell (HUVEC) culture, but supports the growth of other cell types, including hepatocytes, smooth muscle cells, and cardiac myocytes.

Comments Supplement with glutamine prior to use. For vascular endothelial cells, 10 mM glutamine (final concentration) is recommended. For other cell lines, 2 mM glutamine (final concentration) is generally required; however, the optimal concentration is dependent on your cell line of interest.
Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also tested for the ability to support the growth of several different cell lines using both sequential subcultures and plating efficiencies. pH, osmolality, and endotoxin tests are also performed as indicated below.




7.2 to 7.6


260 to 300 mOsm/kg H2O

Sterility Testing



≤ 0.25 EU/mL



Growth Promotion


*Please consult the Certificate of Analysis for lot-specific test results.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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