MEF (CF-1) (ATCC® SCRC-1040)

Cell Type: Fibroblast  /  Tissue: Embryo  / 

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Cell Type Fibroblast
Product Format frozen
Morphology Fibroblast
Culture Properties Adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 14 days gestation embryo
Gender male and female mixed
Strain CF-1, non-inbred mouse strain (non-agouti albino) from Carworth Farms
ATCC has successfully irradiated (SCRC-1040.1) and treated the cells with Mitomycin C (SCRC-1040.2a) for use as a feeder layer.
The cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state.
Storage Conditions liquid nitrogen vapor phase
The cell line was established by ATCC in 2003 from embryonic day 14 (E14) CF-1 mouse embryos.
Clinical Data
male and female mixed
The growth of these cells should be arrested before being used as a feeder layer.
If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no.6 (P6)
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Subculturing Procedure:
    To insure the highest level of viability, be sure to warm media and Trypsin / EDTA to 37┬║C before using it on the cells. Cells should be split when they reach confluency. Split cells at approximately 0.4 X 104 cells/cm2
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 1XPBS (SCRR-2201) solution to remove all traces of serum, which contain trypsin inhibitor.
    3. Add 5 mL of Trypsin-EDTA (0.25% (w/v) Trypsin-0.53 mM EDTA solution, ATCC# 30-2101) solution to flask and incubate for 1 minute, gently tapping the flask observe cells under an inverted microscope until cells detach (usually within 1 to 2 minutes).
    4. Add 6.0 to 8.0 mL of complete growth medium and rinse surface of the flask to detach all cells. Gently pipetting up and down will break cell clumps.
    5. Transfer all cells into a centrifuge bottle or tube and centrifuge at 271x g for 5 minutes.
    6. Remove and discard the supernatant
    7. Add 10 mL complete growth medium to cell pellet and with 10 mL pipette resuspend the cells gently (create a single-cell suspension).
    8. Add more complete growth medium to cell suspension as needed to plate cells.
    9. Place flasks in incubator @ 37°C with a 5% CO2 in air atmosphere.
    Subcultivation Ratio: Plate the cells at approximately of 0.4 X 104 cells/cm2.
    Medium Renewal: Twice a week or when pH decreases.
    Liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Name of Depositor ATCC
    Year of Origin 2003

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.