Acidithiobacillus thiooxidans (Waksman and Joffe) Kelly and Wood

1. Incubate the test tube culture at room temperature for 1 to 2 weeks upon arrival.
2. After the sulfur has settled to the bottom of the test tube examine the culture by phase microscopy to insure growth has occurred.
3. Use the entire tube to inoculate 100 mL of Medium #125 (in a 250 mL screw cap flask) and then place the flask on a shelf at room temperature.
4. Let the flask incubate for 2 to 4 weeks.  Growth is indicated when the sulfur settles to the bottom of the flask.

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